Plan sequencing costs by read target — for RNA-seq, amplicon, and multi-assay service labs
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Before you start
You need at least one sequencer with a flow cell configured in My Lab before this calculator can produce meaningful results.
Not set up yet? Read the My Lab Setup Guide →
FlowSEQ has two ways to specify a sequencing project. The right choice depends on what your protocol spec sheet tells you:
| Single / Multi-Sequencer Calculator | Read-Based Calculator | |
|---|---|---|
| You know… | Genome size + coverage target (e.g. 30×) | Reads per sample target (e.g. 50M reads) |
| Input | Genome selection + coverage + sample count | Sample group + reads/sample |
| Capacity unit | Gb (gigabases of sequence data) | Reads (read count) |
| Output includes | Runs needed, cost/sample | Flow cells needed, cost/sample, cost/million reads |
| Typical use | WGS, WES, targeted enrichment | RNA-seq, ATAC-seq, amplicon, 16S/metagenomics, service core quoting |
✓ Simple rule: If your protocol spec sheet says "50 million reads per sample" or "20M paired-end reads", use this calculator. If it says "30× coverage" or "100× depth", use the Single Sequencer Calculator.